2️⃣Manual Specific Tissue Detection

Distincton between different region within one sample.

Make sure to open the Project_Regions for this part.

Whole bovine disc, shown with the manual detection for each tissue component

1. Add the classes needed to distinguish the regions. Remember if you click on Auto set the class will stay the chosen one once you start drawing regions.

2. Manually draw and classify each region in every slide and save it. Different tools to draw can be used, see what works best for you.

3. Make sure to save the changes to the slides when you switch from one to the other, this ensures that your specific regions stay.

Regions with tissue folds or excessive staining can be excluded from the analysis.

With this manual step, an immunopositivity rate for each tissue region will be the output.